Supply Cunnada/Warshad Cusub Budada Nukliyeerka ah

Sharaxaada Alaabta:

Nuclease waa qayb ka mid ah ensaymes kuwaas oo kicin kara hydrolysis of phosphodiester bonds ee nucleic acid (DNA ama RNA) molecules. Iyadoo ku xiran substrate-yada ay ku shaqeeyaan, nucleases waxaa loo qaybin karaa enzymes DNA (DNase) iyo RNA enzymes (RNase).

Nuclease-yada leh waxqabadka ≥100,000 u/g ayaa ah kuwo aad waxtar u leh iyo diyaarinta enzym-ka oo si weyn loogu isticmaalo bayotechnoolajiyada, daawada, cuntada, ilaalinta deegaanka, iyo qurxinta. Dhaqdhaqaaqooda sare iyo gaar ahaan waxay ka dhigaan enzymes muhiimka ah ee hoos u dhaca acid nucleic iyo wax ka beddelka, oo leh faa'iidooyin dhaqaale iyo deegaan oo muhiim ah. Foomka budada ama dareeraha waa sahlan tahay in la kaydiyo lana raro, waxayna ku habboon tahay codsiyada warshadaha waaweyn.

COA:

Shaqada:

1.Hydrolysis Nucleic Acid Katalytic Heightly Heightly
enzyme DNA:hydrolyzes phosphodiester bond ee molecules DNA si ay u abuuraan oligonucleotides ama mononucleotides.

enzyme RNA:hydrolyzes phosphodiester bond ee molecules RNA si ay u abuuraan oligonucleotides ama mononucleotides.

2.Specificity sare
Iyada oo ku xidhan nooca, waxay si gaar ah ugu dhaqmi kartaa asiidhyada nucleic-ka ah ee hal-xidhaale ah ama laba-geeska ah, ama taxane gaar ah (sida xaddidaadda endonucleases).

3. La qabsiga pH
Waxay muujisaa firfircoonida ugu fiicneed ee hoos timaada aashitada daciifka ah iyo xaaladaha dhexdhexaadka ah (pH 6.0-8.0).

4.Xakamaynta heerkulka
Waxay ku haysaa dhaqdhaqaaq sare gudaha heerkul dhexdhexaad ah (badanaa 37-60 ° C).

5.Xasilloonida
Waxay leedahay xasilooni wanaagsan labadaba dareeraha iyo qaababka adag, oo ku habboon kaydinta muddada dheer iyo gaadiidka.

Codsiga:

Cilmi-baarista Bayoloji
● Injineeriyada hidda-socodka: waxaa loo isticmaalaa goynta, wax ka beddelka iyo dib-u-habaynta DNA/RNA, sida adeegsiga xannibaadaha endonucleases ee hidda-wadaha.
●Tijaabooyin bayooloji molecular: waxaa loo isticmaalaa in lagu saaro wasakhda shaybaarada aashitada nukliyeerka, sida enzymes RNA ee loo isticmaalo in lagu saaro wasakheynta RNA ee shaybaarada DNA-ga.
●Isku xigxiga aashitada nukliyeerka: waxaa loo isticmaalaa in lagu diyaariyo jajabyada aashitada nucleic iyo caawinta isku xigxiga-sare.

Warshadaha dawooyinka
●Wax soo saarka daroogada: loo isticmaalo diyaarinta iyo sifaynta daawooyinka nucleic acid, sida soo saarista tallaallada mRNA.
●Cudurka ogaanshaha: loo isticmaalo sidii reagement ogaanshaha si loo ogaado calaamadaha nucleic acid (sida fayraska RNA/DNA).
●Daawaynta ka hortagga fayraska: waxaa loo isticmaalaa in lagu horumariyo dawooyinka nukliyeerka iyo hoos u dhigista asiidhyada nucleic-ka ee fayraska.

Warshadaha cuntada
●Baaritaannada badbaadada cuntada: waxaa loo isticmaalaa in lagu ogaado faddaraynta microbial ee cuntada (sida bakteeriyada iyo asiidhyada nucleic-ka ee fayraska).
●Cuntada shaqaynaysa: waxaa loo isticmaalaa in lagu soo saaro maaddooyinka shaqaynaysa ee nucleotide si kor loogu qaado qiimaha nafaqada ee cuntada.

Goobta Ilaalinta Deegaanka
Waxaa loo isticmaalay in lagu daweeyo biyaha wasakhda ah ee warshadaha oo ay ku jiraan asiidhyada nucleic-ka iyo hoos u dhigida wasakhowga dabiiciga ah.
●In bioremediation, loo isticmaalo si hoos loogu dhigo wasakhaha nucleic acid ee deegaanka.

Warshadaha qurxinta
●Waxa loo isticmaalaa alaabta daryeelka maqaarka si ay u kala gooyaan qaybaha aashitada nucleic iyo kor u qaadida nuugista iyo shaqada alaabta.
●Sida walax firfircoon u ah horumarinta alaabta ka hortagga gabowga iyo dayactirka.