Icyatsi gishya Gutanga ibiryo / Inganda Urwego Nuclease Ifu
Ibisobanuro ku bicuruzwa:
Nuclease nicyiciro cyimisemburo ishobora guhagarika hydrolysis yumubumbe wa fosifori muri acide nucleic (ADN cyangwa RNA). Ukurikije insimburangingo bakora, nuclease irashobora kugabanywa mumisemburo ya ADN (DNase) na enzymes za RNA (RNase).
Nuclease ifite ibikorwa bya, 000 100.000 u / g ni byiza cyane kandi bihindura imisemburo ya enzyme ikoreshwa cyane mubinyabuzima, ubuvuzi, ibiryo, kurengera ibidukikije, no kwisiga. Ibikorwa byabo byinshi kandi byihariye bituma bakora enzymes zingenzi zo kwangirika kwa acide nucleique no guhindura, hamwe nibyiza byubukungu nibidukikije. Ifu cyangwa ifu byoroshye kubika no gutwara, kandi birakwiriye mubikorwa binini byinganda.
COA ：
| Items | Ibisobanuro | Igisubizos |
| Kugaragara | Ifu yumuhondo yoroheje | Bikubiyemo |
| Impumuro | Impumuro iranga impumuro ya fermentation | Bikubiyemo |
| Igikorwa cya enzyme (nuclease) | , 000 100.000 u / g | Bikubiyemo |
| PH | 6.0-8.0 | 7.0 |
| Gutakaza kumisha | ＜ 5 ppm | Bikubiyemo |
| Pb | ＜ 3 ppm | Bikubiyemo |
| Umubare wuzuye | ＜ 50000 CFU / g | 13000CFU / g |
| E.Coli | Ibibi | Bikubiyemo |
| Salmonella | Ibibi | Bikubiyemo |
| Kudashobora guhinduka | ≤ 0.1% | Yujuje ibyangombwa |
| Ububiko | Ubitswe mu mufuka ufunze imifuka ya poly, ahantu hakonje kandi humye | |
| Ubuzima bwa Shelf | Imyaka 2 iyo ibitswe neza | |
Igikorwa:
1.Byiza cyane Catalitike Nucleic Acide Hydrolysis
Enzyme ya ADN:hydrolyzes ihuza fosifori muri molekile ya ADN kugirango itange oligonucleotide cyangwa mononucleotide.
Enzyme ya RNA:hydrolyzes phosphodiester ihuza muri molekile ya RNA kugirango itange oligonucleotide cyangwa mononucleotide.
2.Ubusobanuro bwihariye
Ukurikije ubwoko, burashobora gukora byumwihariko kuri acide nucleic imwe imwe cyangwa inshuro ebyiri, cyangwa urutonde rwihariye (nko kubuza endonuclease).
3.pH Guhuza n'imihindagurikire y'ikirere
Yerekana ibikorwa byiza munsi ya acide nkeya mubihe bidafite aho bibogamiye (pH 6.0-8.0).
4.Ubushuhe
Ikomeza ibikorwa byinshi mubushyuhe buringaniye (mubisanzwe 37-60 ° C).
5. Guhagarara
Ifite ituze ryiza muburyo bwamazi kandi bukomeye, bubereye kubika igihe kirekire no gutwara.
Gusaba ：
Ubushakashatsi bwibinyabuzima
Engineering Ubwubatsi bwa genetike: bukoreshwa mugukata, guhindura no guhuza ADN / RNA, nko gukoresha endonuclease yo kubuza muri cloni ya gene.
Exper Ubushakashatsi bwa biologiya ya molekuline: bukoreshwa mugukuraho umwanda wintangarugero ya acide nucleic, nka enzymes za RNA zikoreshwa mugukuraho kwanduza RNA mubyitegererezo bya ADN.
Acide Urutonde rwa acide nucleique: rukoreshwa mugutegura ibice bya acide nucleic no gufasha muburyo bukurikiranye.
Inganda zimiti
Production Gukora ibiyobyabwenge: bikoreshwa mugutegura no kweza imiti ya acide nucleique, nko gukora inkingo za mRNA.
Diagn Gusuzuma indwara: bikoreshwa nka reagent yo gusuzuma kugirango umenye ibimenyetso bya aside nucleique (nka virusi RNA / ADN).
Ubuvuzi bwa virusi: bukoreshwa mugutezimbere imiti ya nuclease no gutesha agaciro aside nucleic aside.
Inganda zikora ibiribwa
Testing Kwipimisha umutekano wibiribwa: bikoreshwa mugutahura mikorobe yanduye mubiryo (nka bagiteri na acide nucleic aside).
Food Ibiryo bikora: bikoreshwa mugukora nucleotide yibikoresho bikora kugirango byongere agaciro kintungamubiri yibiribwa.
Umwanya wo Kurengera Ibidukikije
Byakoreshejwe mu gutunganya amazi mabi yinganda arimo acide nucleic no gutesha agaciro imyanda ihumanya.
● Muri bioremediation, ikoreshwa mu kwangiza aside nucleic ihumanya ibidukikije.
Inganda zo kwisiga
● Ikoreshwa mubicuruzwa byita kuruhu kugirango ibore aside nucleic aside kandi yongere ubwinjiriro nibikorwa byibicuruzwa.
● Nibintu byingenzi mugutezimbere ibicuruzwa birwanya gusaza no gusana.
Gupakira & Gutanga
