Nri/ụlọ ọrụ mmepụta ihe nke Newgreen ntụ ntụ ntụ
Nkọwa ngwaahịa:
Nuclease bụ klas nke enzymes nke nwere ike ime ka hydrolysis nke njikọ phosphodiester dị na nucleic acid (DNA ma ọ bụ RNA). Dabere n'ụdị ihe ha na-eme, enwere ike kewaa nucleases n'ime enzymes DNA (DNase) na RNA enzymes (RNase).
Nucleases nwere ọrụ nke ≥100,000 u/g na-arụ ọrụ nke ọma na nkwadebe enzyme dị iche iche nke a na-ejikarị na biotechnology, ọgwụ, nri, nchebe gburugburu ebe obibi, na ịchọ mma. Ọrụ ha dị elu na nkọwapụta na-eme ka ha bụrụ isi enzymes maka mmebi na ngbanwe nke acid nucleic, nwere uru dị mkpa akụ na ụba na gburugburu ebe obibi. Ụdị ntụ ntụ ma ọ bụ mmiri mmiri dị mfe ịchekwa na ibufe, ọ dịkwa mma maka ngwa ọrụ mmepụta ihe buru ibu.
COA:
| Item | Nkọwapụta | Nsonaazụs |
| Ọdịdị | Ntụ ntụ edo edo dị ọkụ | Na-akwado |
| Isi | Njirimara njirimara nke ísì gbaa ụka | Na-akwado |
| Ọrụ nke enzyme (nuclease) | ≥100,000 u/g | Na-akwado |
| PH | 6.0-8.0 | 7.0 |
| Ọnwụ na ihicha | 5 ppm | Na-akwado |
| Pb | 3 ppm | Na-akwado |
| Ngụkọta ọnụ ọgụgụ efere | 50000 CFU/g | 13000CFU/g |
| E.Coli | Ihe ọjọọ | Na-akwado |
| Salmonella | Ihe ọjọọ | Na-akwado |
| Enweghi ike | ≤0.1% | tozuru oke |
| Nchekwa | A na-echekwa ya na akpa poly siri ike, na ebe dị mma na nke kpọrọ nkụ | |
| Ndụ nchekwa | Afọ 2 mgbe echekwara nke ọma | |
Ọrụ:
1.Highly dị irè Catalytic Nucleic Acid Hydrolysis
DNA enzyme:hydrolyzes phosphodiester bond na DNA molecules iji mepụta oligonucleotides ma ọ bụ mononucleotides.
RNA enzyme:hydrolyzes phosphodiester bond na ụmụ irighiri RNA iji mepụta oligonucleotides ma ọ bụ mononucleotides.
2.High Specificity
Dabere n'ụdị ahụ, ọ nwere ike ime ihe kpọmkwem na nucleic acids nke nwere otu eriri ma ọ bụ okpukpu abụọ, ma ọ bụ usoro dị iche iche (dị ka mmachi endonucleases).
3.pH ndakọrịta
Na-egosipụta ọrụ kachasị mma n'okpuru ọnọdụ acid na-adịghị ike na-anọpụ iche (pH 6.0-8.0).
4.Thermotolerance
Na-arụ ọrụ dị elu n'ime oke okpomọkụ (na-emekarị 37-60 ° C).
5.Stability
Ọ nwere nkwụsi ike dị mma na ma mmiri mmiri na ụdị siri ike, nke kwesịrị ekwesị maka nchekwa na njem ogologo oge.
Ngwa:
Nchọpụta biotechnology
● Injinịa mkpụrụ ndụ ihe nketa: ejiri ya maka ịkpụ, gbanwee na ijikọta DNA / RNA, dị ka itinye ihe mgbochi endonucleases na mkpụrụ ndụ ihe nketa.
●Nnwale ihe ndị dị ndụ: a na-eji ewepụ mmetọ n'ime sample nucleic acid, dị ka enzymes RNA iji wepụ mmetọ RNA na nlele DNA.
●Nucleic acid sequencing: eji akwadebe iberibe nucleic acid ma nyere aka n'usoro dị elu.
Ụlọ ọrụ ọgwụ
● Mmepụta ọgwụ: eji maka nkwadebe na sachaa ọgwụ nucleic acid, dị ka imepụta ọgwụ mgbochi mRNA.
●Nchọpụta ọrịa: a na-eji dị ka ihe na-eme nchọpụta nchọpụta iji chọpụta akara nucleic acid (dị ka nje RNA/DNA).
●Ọgwụ mgbochi nje: eji emepụta ọgwụ nuclease na iweda acid nucleic viral.
Ụlọ ọrụ nri
●Nnwale nchekwa nri: eji achọpụta mmetọ microbial na nri (dị ka nje bacteria na nje nucleic acid).
●Nri na-arụ ọrụ: eji emepụta ihe na-arụ ọrụ nucleotide iji kwalite uru nri nri.
Ubi nchekwa gburugburu
● A na-eji agwọ mmiri mkpofu ụlọ ọrụ nwere acid nucleic ma na-emebi ihe ndị na-emetọ ihe.
●Na bioremediation, a na-eji emebi ihe mmetọ nucleic acid na gburugburu ebe obibi.
Ụlọ ọrụ ịchọ mma
● A na-eji ngwaahịa na-elekọta anụ ahụ na-emepụta ihe ndị dị n'ime acid nucleic ma welie ntinye na ọrụ nke ngwaahịa.
●Dị ka ihe na-arụsi ọrụ ike na mmepe nke mgbochi ịka nká na nrụzi ngwaahịa.
Ngwungwu & Nbunye
