I-Bacillus licheniformis: "Umgcini wohlaza" wezoLimo kunye noShishino

●Yintoni i Bacillus licheniformis?

Njengeentlobo zeenkwenkwezi zohlobo lweBacillus,Bacillus licheniformis,ngokuguquguquka okuqinileyo kokusingqongileyo kunye nobuchule obuguquguqukayo bemetabolism, iba ngumthombo ongundoqo we-microbial oqhuba inguqu yezolimo eluhlaza, imveliso yemizi-mveliso ecocekileyo, kunye nokhathalelo lwezempilo. Iipropati zayo ezizodwa zebhayoloji kunye nosetyenziso olubanzi lufumene ingqalelo ebalulekileyo kwihlabathi jikelele.

Bacillus licheniformisukwiklasi yeBacillus, phylum Firmicutes. Yintsholongwane yeGram-positive enomzimba omile okwentonga (0.8×1.5-3.5μm) eyenza i-elliptical mesozoic spores. Iyakwazi ukumelana nobushushu obuphezulu (ukusinda imizuzu emininzi kwi-100°C), i-asidi kunye ne-alkali (pH 3.0-9.8), kunye netyuwa ephezulu (≤I-10% ye-NaCl). I-metabolites yayo ibandakanya i-lipopeptide antibiotics, i-chitinases, kunye ne-analog ye-hormone yezityalo, ebonisa i-antimicrobial, ukukhula-ukukhuthaza, kunye neempawu zokulungisa umhlaba. Njenge "injineli yendalo" yendalo, inqanda i-pathogens ngokusebenzisa i-oksijini ye-biological ye-biological ngelixa ikhuthaza ukwanda kwee-microorganisms eziluncedo kunye nokugcina ibhalansi ye-microbial.

●Yintoni naIingeneloKwe Bacillus licheniformis ?

1. Ulawulo lwebhayoloji: Ngokukhupha iipeptide zeantimicrobial (ezifana ne-surfactin) kunye nokukhuphisana ngokuhlala kwi-ecological niches, inqanda iintsholongwane ezifumaneka emhlabeni ezifana neFusarium kunye ne-Rhizoctonia, ukuphumeza umlinganiselo we-60% -87% wokulawula ngokuchasene nengqolowa ukuthatha-zonke izifo kunye ne-cucumber downy.

2. Ukukhuthazwa kokuKhula: Idibanisa i-indoleacetic acid (IAA) kunye ne-cytokinins, ivuselela ukukhula kweengcambu zezityalo, kwaye iphucula ukufunyanwa kwe-nitrogen kunye ne-phosphorus, inokunyusa irayisi kunye nezivuno zengqolowa nge-8% -12%.

3. Ukulungiswa kokusiNgqongileyo: Yehlisa isidima iintsalela zezitshabalalisi (isusa ngaphezu kwe-90% ye-organophosphorus), ifunxa isinyithi esinzima (ilothe necadmium), kwaye ilungisa umhlaba ongcolileyo. Iminyaka emithathu yokusetyenziswa ngokuqhubekayo inokunyusa i-porosity yomhlaba nge-15%.

4. Ukuphuculwa kweShishini: Ivelisa i-alkaline protease (i-akhawunti ye-50% yemveliso ye-enzyme yehlabathi) kunye ne-amylase yokusetyenziswa kwi-detergents kunye nokulungiswa kokutya. Ikwabilisa kwaye ivelise i-antibiotics efana ne-bacitracin, ithatha indawo yeenkqubo ze-chemical synthesis.

●Yintoni naIsiceloOf Bacillus licheniformis?

1. Ezolimo: I-Biopesticides, i-conditioners yomhlaba, izongezo zokutya

2. Ukufuywa kwezilwanyana: Iiprobiotics (izongezo zempilo yamathumbu), iinkcubeko ze-silage starter. Ukongeza i-0.1% -0.3% kwisondlo kunciphisa urhudo kwaye kuphucula ukuguqulwa kwesondlo.

3. Amachiza: Iipilisi zebhaktheriya eziphilileyo (zonyango lwe-enteritis), i-nanocarriers (ukuhanjiswa kweziyobisi ezijoliswe kuyo),Bacillus licheniformisiicapsules zebhaktheriya eziphilayo (i-250 yezigidi ze-CFU / i-capsule) zilawula i-intestinal flora.

4. Ukhuseleko lokusiNgqongileyo: Ukucocwa kwamanzi amdaka (ukonakaliswa kwe-ammonia nitrogen), isicoci sebhayoloji sokuhlanjwa kwempahla (ukucoca iprotease). Ukusebenzisa i-50-100g / mu (malunga ne-1.5 iihektare) kucoca amanzi e-aquaculture, ukunciphisa i-ammonia nitrogen ukusuka kwi-10mg / L ukuya kwi-2mg / L.

1. Umzi-mveliso: I-Biofuels (ethanol), i-nanomaterials (ukwenziwa kweenanocubes zegolide)

●Umthamo kunye nezikhokelo zoKhuseleko of Bacillus licheniformis

1. Izicelo zezoLimo

Ukunyangwa komhlaba: 50-100 g / mu, xuba nomhlaba kunye nokusasazwa, okanye uhlambulule ngamanzi ukunkcenkceshela iingcambu;

I-Seed Coating: I-1 yebhiliyoni ye-CFU/imbewu yokuphucula izinga lokuntshula;

I-Feed Additive: 0.1% -0.3% (ixesha lokutyeba) okanye i-0.02% -0.03% (imfuyo encinci).

2. Ukusetyenziswa kwezonyango

Ukuqulunqwa komlomo: Abantu abadala: ii-capsules ze-2 (0.25 g / ipilisi) ngamaxesha e-3 imihla ngemihla; Abantwana: 50% kwisisu esingenalutho;

Ukuqulunqwa kwezihloko: I-Suppository ye-Vaginal (i-1 yebhiliyoni ye-CFU / i-suppository), kanye ngosuku ngeentsuku ezi-7 ezilandelelanayo.

3. Ukuvutshelwa kweShishini

I-Liquid Fermentation: Ubushushu be-37-45 ° C, i-pH 7.0, i-oxygen echithwe ≥ 20%. Yongeza nge-0.5% yotywala bombona ukunyusa ukusebenza kakuhle kwemveliso ye-enzyme.

I-Solid-State Fermentation: I-Corncob substrate, i-50% -60% yokufuma, ukwandisa umsebenzi we-protease ngama-30%. Iingcebiso zoKhuseleko:

Gwema ukuxuba kunye nee-oxidants ezinamandla kunye namalungiselelo obhedu. Igranulation yobushushu obuphezulu kufuneka iqinisekise izinga lokusinda kwembewu>85%.

Ukusetyenziswa kwezonyango, i-antibiotics kufuneka ilawulwe iiyure ezintathu ngaphandle. Sebenzisa ngononophelo kwabo bane-allergies.

Kwizicelo zokusingqongileyo, bambelela kwizikhokelo zedosi; ukusetyenziswa ngokugqithisileyo kunokubangela ukungalingani kwendalo.

● Newgreen Supply High Quality Bacillus licheniformis Umgubo